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Get PriceAbstract. A pilot experiment for the construction of a hamster derived, high producer cell line using site specific recombination is described. In the experiment chromosomal loci with intrinsic high expression characteristics were sought via infection with a retroviral construct, containing double FRT sites and subsequent screening for overproduction of an encoded markergene.
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Get PriceThe acc(6')-Ib-cr and qnrB1 plasmid-mediated quinolone resistance genes were also detected in some of the isolates. This is the first report on the emergence and spread of CTX-M-producing E. coli in the community of the Czech Republic, indicating the high prevalence of ST131 clone among CTX-M producers. PMID: 25188031 [Indexed for MEDLINE]
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Get PriceBy contrast, plasmids in P. purpureum occur at high copy numbers of approximately 20 copies per cell (i.e., 10 times higher copy numbers than those of the chromosomes, considering that the ...
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Get PriceHere we describe a straightforward screening platform based on the creation of tailor-made expression plasmids by Golden Gate cloning without combinatorial limitations [23, 24].We defined six classes of plasmid features that are combined into expression plasmids in a single step (Fig. 1): the promoter system, two possible fusion partners, the protease cleavage site, the target protein and the ...
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Get PriceDiversity of Plasmids and Genes Encoding Resistance to Extended-Spectrum -Lactamase in Escherichia coli from Different Animal Sources Abasiofiok Ibekwe 1,*, Lisa Durso 2, Thomas F. Ducey 3, Adelumola Oladeinde 4, Charlene R. Jackson 4, Jonathan G. Frye 4, Robert Dungan 5, Tom Moorman 6, John P. Brooks 7, Amarachukwu Obayiuwana 8,
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